Auto annotation solves both of these common problems by allowing you to scan a sequence against a folder containing a collection of existing annotated sequences and MacVector will find the matchingfeatures in the folder and add those to the starting sequence. Once identified you can quickly save each intron or exon as a feature on the parental sequence.Īuto Annotation allows you to quickly annotate a plain unannotated sequence, or to modify downloaded sequences to match the carefully curated graphical appearance of your favorite genes. Although the effect the exon-intron GC content difference has in a short intronic environment is unclear, the fact that we did not change the splicing mode (according to intron length Talerico and Berget, 1994), but did abolish the exon-intron GC content difference, may suggest its involvement in the intron definition mechanism as well. Then repeat the process to answer the following questions about the 5’ splice donor and 3’ splice acceptor sites for the tra-RA (female specific) intron 2. ![]() Actual splice sites at ambiguous junctions are determined using the standard GT.AG intron rule. We can use the same approach to map the exon-intron boundaries of the second intron. MacVector allows you to align cDNA clones against a corresponding genomic sequence and automatically identify the introns and exons. The following videos show more features of MacVector (Note: if you experience jerky playback, click pause and wait until the video downloads fully before playback).ĬDNA Alignments and Splice Site Identification
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